By the methods of the scenario-based questionnaire survey, this paper sent questionnaires to the regulation departments and other market participants. According the data collected through the questionnaires, this paper does an empirical analysis to the irrational behavior of the regulation departments and market participants.Our results shows that the 11 bias of representative prejudice, anchor and adjustment effect, confirmative bias, framing traps, over-confidence, loss-aversion, regret-aversion, handling/disposing Effect, sink-cost bias, mental accounting and ambiguity-aversion commonly exist, which affect and constraint their acts and prevent them from making the correct and reasonable decisions. As for the effect of the irrationlity on the regulation,the sequence of the bias in terms of their significance are:regrat-aversion,anchor and adjustment effect,coss-aversion,handing effect,framing effect,psychological accounts,representative prejudice,ambiguity-aversion,over-comfidence. The paper points out the irrational behaviors of regulators and other market participants and calls the attentions of regulators of their own irrationality and the consequent safeguards and of adequate consideration of other market participants' irrationality.